Constructing and Screening a Recombinant DNA Library

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Session Overview


This session will review how to make a recombinant genomic DNA library and how to use this library to find a specific gene. This session will outline using a library to clone a gene by complementation of a mutant phenotype.

Learning Objectives

  • To understand what a recombinant genomic DNA library is and how it is constructed.
  • To identify how to use a genomic library to identify a gene of interest.
  • To understand how to clone the gene of interest by complementation of a mutant phenoptype.
  • To understand the limitations of a genomic library.

Session Activities

Lecture Video

Watch the lecture video excerpt

Check Yourself



Session Activities

Help Session Video

Watch the short video of Robert Dorkin explaining genomic libraries and cDNA libraries.

Practice Problems

Further Study

Useful Links


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